Hybrid nanopores are a type of nanopore sequencing method that combines both biological and solid-state components to sequence DNA or RNA molecules.
These nanopores are engineered to incorporate both biological pores, typically derived from proteins like α-hemolysin, and solid-state materials like graphene or silicon nitride.
Advantages of Hybrid Nanopores:
- Improved Control: Hybrid nanopores offer greater control over pore size and structure, allowing for tailored sequencing applications. They can be engineered to accommodate various types of molecules, making them versatile.
- Enhanced Sensitivity: The combination of biological and solid-state components can enhance the sensitivity of nanopore sequencing. This improved sensitivity allows for the detection of smaller or modified DNA or RNA molecules.
- Longer Sequences: Hybrid nanopores may enable longer read lengths compared to solely biological nanopores. Longer reads are advantageous in assembling complex genomes and resolving repetitive sequences.
- Reduced Blockages: Biological pores are susceptible to blockages from DNA or RNA molecules. Hybrid nanopores can mitigate this issue by using solid-state segments in conjunction with biological pores, improving the overall efficiency of the sequencing process.
- Customization: Researchers can tailor the properties of hybrid nanopores to suit specific sequencing requirements, such as optimizing speed, accuracy, or detection of specific types of modifications (e.g., epigenetic marks).
- Compatibility: Hybrid nanopore sequencing can be integrated into existing nanopore platforms, facilitating the adoption of this technology in research and clinical settings.
While hybrid nanopores offer several advantages, it’s important to note that they are still an evolving technology, and ongoing research is focused on optimizing their performance and reducing cost.